Hyalgan
THE PROMINENT ROLE OF HYALURONAN
Punzi L. et al.
Clin Exp Rheumatol 1989; 7 (3) : 247-50
Studies outline: to investigate the role of intra-articular HA on PGE2 and cAMP concentration in the synovial fluid.
In patients affected with knee-joint effusion due to various arthropathies and randomly allocated to the treatment with intra-articular injection of Hyalgan, a significant reduction of the synovial fluid volume and prostaglandin PGE2 was found, whereas cAMP concentration was significantly increased. These data could suggest an anti-inflammatory effect of HA that appears to be mediated by PG-inhibition as well as cAMP stimulation.
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Brun P. et al.
Osteoarthritis and Cartilage 2003; 11 (3) : 208-16
Studies outline to investigate the role of hyaluronan on the viability of normal human chondrocytes mediated by the CD44, after oxidative cell injury.
Direct addition of Hyalgan (HA 500-730 KDa) significantly increased cell survival in "normal" chondrocyte primary cultures. Similarly, addition of this same dose of HA to cultures of free radical-damaged chondrocytes, restored the viability to baseline conditions.
Cell viability rates dropped significantly when CD44 receptor binding was inhibited, indicating that cell growth was mediated by the CD44 receptor.
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Julovi S.M. et al.
Arthritis Rheum 2004; 50 (2) : 516-25
Study's outline: to investigate the mechanism of the inhibitory action of hyaluronan (HA) on interleukin - 1 beta (IL-1 beta) - stimulated production of matrix metalloproteinases (MMPs) in human articular cartilage.
"Normal" and OA cartilage explant cultures were incubated with a clinically used form of 800 KDa hyaluronan (HA).
Treatment with 800 KDa HA resulted in significant suppression of IL - 1 beta-stimulated production of MMPs 1, 3, and 13. The action of HA on IL-1 beta may involve direct interaction between HA and CD44 on chondrocytes.
The study supports the clinical use of HA on the treatment of OA.
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Maneiro E. et al.
Clin Exp Rheumatol 2004; 22 (3) : 307-12
Study's outline: to analyze the impact of molecular weight (MW) on the biological effects of HA on human OA chondrocytes.
The in vitro biological effects of Hyalgan (HA 500-730 KDa) on human OA chondrocytes were compared to those of another commercial preparation of different molecular weight (Hylan GF-20). Hyalgan reduced the synthesis of both IL - 1 - induced NO and PGE2 by 70% and 45% respectively. Furthermore, Hyalgan decreased the apoptosis induced by SNP to 40%, as compared to 36% of the higher molecular weight preparation.
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